NPPC Kit ELISA
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- Antigène Voir toutes NPPC Kits ELISA
- NPPC (Natriuretic Peptide Type C (NPPC))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human C-type Natriuretic Peptide/CNP ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human C-type Natriuretic Peptide.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 6 months
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- Antigène Voir toutes NPPC Kits ELISA
- NPPC (Natriuretic Peptide Type C (NPPC))
- Autre désignation
- C-type Natriuretic Peptide (CNP (NPPC Produits)
- Synonymes
- CNPF Kit ELISA, CNPI Kit ELISA, CNPII Kit ELISA, Cnp1 Kit ELISA, CNP Kit ELISA, lbab Kit ELISA, CNP2 Kit ELISA, CNP3 Kit ELISA, cnp Kit ELISA, nppc Kit ELISA, 2',3'-cyclic nucleotide 3' phosphodiesterase Kit ELISA, natriuretic peptide type C Kit ELISA, natriuretic peptide C Kit ELISA, natriuretic peptide C S homeolog Kit ELISA, Cnp Kit ELISA, Nppc Kit ELISA, NPPC Kit ELISA, nppc.S Kit ELISA
- ID gène
- 4880
- UniProt
- P23582
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