LTBP2 Kit ELISA

N° du produit ABIN5690903

Détail du produit LTBP2 Kit ELISA

Antigène: LTBP2 (Latent Transforming Growth Factor beta Binding Protein 2 (LTBP2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Latent TGF-beta bp2/LTBP-2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Latent TGF-beta bp2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: LTBP2 (Latent Transforming Growth Factor beta Binding Protein 2 (LTBP2))

Autre désignation: Latent TGF-beta bp2 (LTBP-2 (LTBP2 Produits)

Synonymes: C14orf141 Kit ELISA, GLC3D Kit ELISA, LTBP3 Kit ELISA, MSPKA Kit ELISA, MSTP031 Kit ELISA, WMS3 Kit ELISA, AW208642 Kit ELISA, LTBP2 Kit ELISA, latent transforming growth factor beta binding protein 2 Kit ELISA, latent transforming growth factor beta binding protein 3 Kit ELISA, LTBP2 Kit ELISA, Ltbp2 Kit ELISA, LTBP3 Kit ELISA, ltbp2 Kit ELISA

Pathways: Growth Factor Binding