MYD88 Kit ELISA

N° du produit ABIN5690914

Détail du produit MYD88 Kit ELISA

Antigène: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human MyD88 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human MyD88.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Autre désignation: MyD88 (MYD88 Produits)

Synonymes: MYD88D Kit ELISA, XMyD88 Kit ELISA, myd88d Kit ELISA, MGC84928 Kit ELISA, CG2078 Kit ELISA, DMMYD88 Kit ELISA, DmMyD88 Kit ELISA, DmMyd88 Kit ELISA, Dmel\\CG2078 Kit ELISA, EP(2)2535 Kit ELISA, Kra Kit ELISA, LD20892 Kit ELISA, MYD88 Kit ELISA, MyD88 Kit ELISA, Myd88F Kit ELISA, dMyD88 Kit ELISA, dMyd88 Kit ELISA, kra Kit ELISA, myd88 Kit ELISA, zgc:103541 Kit ELISA, GB12344 Kit ELISA, NV10640 Kit ELISA, myd88-a Kit ELISA, myd88-b Kit ELISA, myeloid differentiation primary response 88 Kit ELISA, myeloid differentiation primary response 88 S homeolog Kit ELISA, myeloid differentiation primary response gene 88 Kit ELISA, CG2078 gene product from transcript CG2078-RB Kit ELISA, myeloid differentiation primary response protein MyD88-A Kit ELISA, myeloid differentiation primary response protein MyD88 Kit ELISA, myeloid differentiation factor 88 Kit ELISA, myeloid differentiation primary response 88 L homeolog Kit ELISA, MYD88 Kit ELISA, myd88.S Kit ELISA, Myd88 Kit ELISA, myd88 Kit ELISA, LOC413194 Kit ELISA, LOC100118553 Kit ELISA, LOC575153 Kit ELISA, myd88.L Kit ELISA

ID gène: 4615

UniProt: Q99836

Pathways: Signalisation NF-kappaB, Signalisation TLR, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades