PAG1 Kit ELISA

N° du produit ABIN5690926

Détail du produit PAG1 Kit ELISA

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PAG1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human PAG1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Autre désignation: PAG1 (PAG1 Produits)

Synonymes: Cbp Kit ELISA, F730007C19Rik Kit ELISA, Pag Kit ELISA, CBP Kit ELISA, PAG Kit ELISA, MGC80192 Kit ELISA, phosphoprotein associated with glycosphingolipid microdomains 1 Kit ELISA, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 Kit ELISA, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog Kit ELISA, Pag1 Kit ELISA, PAG1 Kit ELISA, pag1.L Kit ELISA, pag1 Kit ELISA

ID gène: 55824

UniProt: Q9NWQ8

Pathways: Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events