PKC zeta Kit ELISA

N° du produit ABIN5690935

Détail du produit PKC zeta Kit ELISA

Antigène: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PKC-zeta ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human PKC-zeta.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Autre désignation: PKC-zeta (PRKCZ Produits)

Synonymes: TPKC Kit ELISA, PKCzeta Kit ELISA, prkcz-A Kit ELISA, pkc-zeta Kit ELISA, PRKCZ Kit ELISA, pkc2 Kit ELISA, PKC-ZETA Kit ELISA, PKC2 Kit ELISA, AI098070 Kit ELISA, C80388 Kit ELISA, Pkcz Kit ELISA, R74924 Kit ELISA, aPKCzeta Kit ELISA, zetaPKC Kit ELISA, 14-3-3-zetaisoform Kit ELISA, r14-3-3 Kit ELISA, pkcz Kit ELISA, si:ch211-150o23.4 Kit ELISA, protein kinase C zeta L homeolog Kit ELISA, protein kinase C zeta Kit ELISA, protein kinase C, zeta Kit ELISA, prkcz.L Kit ELISA, PRKCZ Kit ELISA, prkcz Kit ELISA, Prkcz Kit ELISA

ID gène: 5590

UniProt: Q05513

Pathways: Signalisation NF-kappaB, Signalisation RTK, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling