SLC32A1 Kit ELISA

N° du produit ABIN5690971

Détail du produit SLC32A1 Kit ELISA

Antigène: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human VIAAT/SLC32A1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human VIAAT.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Autre désignation: VIAAT (SLC32A1 (SLC32A1 Produits)

Synonymes: VGAT Kit ELISA, VIAAT Kit ELISA, vGAT Kit ELISA, Ci-vGAT Kit ELISA, slc32a1 Kit ELISA, viaat Kit ELISA, MGC68938 Kit ELISA, vgat Kit ELISA, xVIAAT Kit ELISA, R75019 Kit ELISA, Viaat Kit ELISA, CG8394 Kit ELISA, CG8394.2 Kit ELISA, Dmel\\CG8394 Kit ELISA, Vgat Kit ELISA, zgc:158324 Kit ELISA, solute carrier family 32 member 1 Kit ELISA, vesicular GABA transporter Kit ELISA, solute carrier family 32 member 1 S homeolog Kit ELISA, vesicular inhibitory amino acid transporter Kit ELISA, Vesicular inhibitory amino acid transporter Kit ELISA, solute carrier family 32 (GABA vesicular transporter), member 1 Kit ELISA, Vesicular GABA Transporter Kit ELISA, SLC32A1 Kit ELISA, vgat Kit ELISA, Bm1_20950 Kit ELISA, slc32a1.S Kit ELISA, slc32a1 Kit ELISA, CpipJ_CPIJ002704 Kit ELISA, viaat Kit ELISA, Slc32a1 Kit ELISA, VGAT Kit ELISA, Tsp_04599 Kit ELISA

ID gène: 140679

UniProt: Q9H598

Pathways: Proton Transport