FGF2 Kit ELISA

N° du produit ABIN577104

Le kit ELISA FGF2 (ABIN577104) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain FGF2.

Aperçu rapide pour FGF2 Kit ELISA (ABIN577104)

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Détail du produit FGF2 Kit ELISA

Fonction: This bFGF enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre- coated with a monoclonal specific for bFGF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. bFGF if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound bFGF and other components of sample. In order to quantitative the amount of bFGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for bFGF is added to each well to

Analytical Method: Quantitative

Sensibilité: The minimum detectable dose of bFGF was determined by adding two standard deviations to the mean optical density value of the 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 80 pg/mL and using Calibrator Diluent II is 78 pg/mL.

Ingrédients: Standards: 1 set/2 vials

Information d'application

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Agent conservateur: Without preservative

Détail du antigène

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Autre désignation: Basic Fibroblast Growth Factor (b-FGF)

Sujet: Human Anti-Mouse Antibody (HAMA) are antibodies found in human serum which have the ability to bind to mouse immunoglobulin G (IgG). The presence of HAMA is the result of an immune reaction following an exposure to mice or other similar agent, which was able to induce the immune system to generate an antibody able to bind to mouse IgG. HAMA is commonly found in patients following treated with mouse monoclonal antibodies associated with some therapeutic or diagnostic procedures. Some human auto-antibodies, the most common of which is rheumatoid factor (Rf), by virtue of their cross reactivity are able to bind mouse IgG. The HAMA assay measures HAMA across a wide dynamic range and it is not necessary to predilute human serum samples before the HAMA ELISA testing

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins