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Thiol Detection System

D
N° du produit ABIN577691
  • Application
    Detection (D)
    Marque
    ThioStar®
    Attributs du produit
    The ThioStar® Thiol Detection Substrate allows users to accurately determine the extent of free thiol content in samples. ThioStar is converted to a brightly fluorescent product upon reaction with the thiol in the sample. The thiol in the sample can either be one that is generated by a reaction, such as the end product of an enzymatic reaction such as glutathione, or can be the cysteine content of the protein to be measured. Free thiols in biological systems have important roles. Oxidatively modified thiol groups of cysteine residues are known to modulate the activity of a growing number of proteins. One of the most pressing problems with this approach is to accurately determine the extent of modification of specific amino acids, such as cysteine residues, in a complex protein sample.
    Almost No Background RAPID - Reacts with Free SH groups in Minutes ADAPTABLE - Works in a Wide Range of Buffers
  • Restrictions
    For Research Use only
  • Stock
    4 °C/-20 °C
    Stockage commentaire
    Store reconstituted at -20°C in the shipping desiccator for long term storage.
  • Calderón, Ortiz-Espín, Iglesias-Fernández, Carbonero, Pallardó, Sevilla, Jiménez: "Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture." dans: Redox biology, Vol. 11, pp. 688-700, (2017) (PubMed).

    Di Nottia, Masciullo, Verrigni, Petrillo, Modoni, Rizzo, Di Giuda, Rizza, Niceta, Torraco, Bianchi, Santoro, Bentivoglio, Bertini, Piemonte, Carrozzo, Silvestri: "DJ-1 modulates mitochondrial response to oxidative stress: clues from a novel diagnosis of PARK7." dans: Clinical genetics, (2016) (PubMed).

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