NGFR Kit ELISA

N° du produit ABIN625340

Détail du produit NGFR Kit ELISA

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 80 pg/mL-20 ng/mL

Seuil minimal de détection: 80 pg/mL

Application: ELISA

Fonction: Human NGFR (TNFRSF16) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensibilité: 80 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour NGFR Kit ELISA (ABIN625340)

Chiang, Kan, Hsiao, Tang, Hsieh, Chiang, Tsai, Yeh, Hsieh, Liang, Chen, Yu: "Bone Marrow Stromal Antigen 2 Is a Novel Plasma Biomarker and Prognosticator for Colorectal Carcinoma: A Secretome-Based Verification Study." dans: Disease markers, Vol. 2015, pp. 874054, (2015) (PubMed).

Détail du antigène

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Autre désignation: NGF R (NGFR Produits)

Synonymes: ngfr Kit ELISA, wu:fb38c02 Kit ELISA, wu:fc10h01 Kit ELISA, CD271 Kit ELISA, Gp80-LNGFR Kit ELISA, TNFRSF16 Kit ELISA, p75(NTR) Kit ELISA, p75NTR Kit ELISA, LNGFR Kit ELISA, RNNGFRR Kit ELISA, Tnfrsf16 Kit ELISA, p75 Kit ELISA, NRH1b Kit ELISA, fullback Kit ELISA, nrh1 Kit ELISA, p75ntr Kit ELISA, tnfrsf16 Kit ELISA, p75NGFR Kit ELISA, nerve growth factor receptor Kit ELISA, neurotrophin receptor associated death domain Kit ELISA, nerve growth factor receptor S homeolog Kit ELISA, nerve growth factor receptor (TNFR superfamily, member 16) Kit ELISA, NGFR Kit ELISA, nradd Kit ELISA, Ngfr Kit ELISA, ngfr.S Kit ELISA

Sujet: Gene Names: NGFR TNFRSF16
Protein names: Tumor necrosis factor receptor superfamily member 16 (Gp80-LNGFR) (Low affinity neurotrophin receptor p75NTR) (Low-affinity nerve growth factor receptor) (NGF receptor) (p75 ICD) (CD antigen CD271)

ID gène: 4804

UniProt: P08138

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding