IL-3 Kit ELISA

N° du produit ABIN6719916

N° du produit (Fournisseur): koa0218

Ce kit ELISA Humain IL-3 est un kit ELISA Colorimetric conçu pour quantifier Humain IL-3.

Aperçu rapide pour IL-3 Kit ELISA (ABIN6719916)

Antigène: IL-3 (Interleukin 3 (IL-3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Détail du produit IL-3 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

N° du produit (Fournisseur): koa0218

Fournisseur: Rockland

Fonction: Human IL-3 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human IL-3. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. It is recommended that each human IL-3 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: A20-F152

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: IL-3

Sujet:

Synonyms: Burst promoting activity, Colony stimulating factor multiple, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL 3, IL-3, IL3, IL3_HUMAN, Interleukin 3, Interleukin-3, Mast cell growth factor, MCGF, Multi CSF, Multilineage colony stimulating factor Multipotential colony stimulating factor, Multipotential colony-stimulating factor, P cell stimulating factor, P-cell-stimulating factor, Thy1 inducing factor

Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

ID gène: 3562

NCBI Accession: NP_000579

UniProt: P08700

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy