MEK2 Kit ELISA

N° du produit ABIN6730501

Détail du produit MEK2 Kit ELISA

Antigène: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human MEK2/MAP2K2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human MEK2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Autre désignation: MEK2 (MAP2K2 (MAP2K2 Produits)

Synonymes: CFC4 Kit ELISA, MAPKK2 Kit ELISA, MEK2 Kit ELISA, MKK2 Kit ELISA, PRKMK2 Kit ELISA, AA589381 Kit ELISA, MK2 Kit ELISA, Prkmk2 Kit ELISA, ATMKK2 Kit ELISA, F27B13.50 Kit ELISA, F27B13_50 Kit ELISA, MAP KINASE KINASE 1 Kit ELISA, MAP kinase kinase 2 Kit ELISA, MK1 Kit ELISA, map2k2 Kit ELISA, wu:fa56c06 Kit ELISA, zgc:123171 Kit ELISA, zgc:172250 Kit ELISA, mitogen-activated protein kinase kinase 2 Kit ELISA, mitogen activated protein kinase kinase 2 Kit ELISA, MAP kinase kinase 2 Kit ELISA, mitogen-activated protein kinase kinase 2a Kit ELISA, mitogen-activated protein kinase kinase 2b Kit ELISA, MAP2K2 Kit ELISA, Map2k2 Kit ELISA, MKK2 Kit ELISA, map2k2a Kit ELISA, map2k2b Kit ELISA

UniProt: P36507

Pathways: Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling