BNIP3L/NIX Kit ELISA

N° du produit ABIN6951385

Détail du produit BNIP3L/NIX Kit ELISA

Antigène: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human BNIP3L ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Autre désignation: BNIP3L (BNIP3L Produits)

Synonymes: BNIP3a Kit ELISA, NIX Kit ELISA, NIP3 Kit ELISA, Nix Kit ELISA, Nip3 Kit ELISA, C86132 Kit ELISA, D14Ertd719e Kit ELISA, Nip3L Kit ELISA, bnip3l Kit ELISA, nip3 Kit ELISA, zgc:110478 Kit ELISA, bnip3l2 Kit ELISA, wu:fb52b03 Kit ELISA, wu:fc02h04 Kit ELISA, BCL2 interacting protein 3 like Kit ELISA, BCL2 interacting protein 3 Kit ELISA, BCL2/adenovirus E1B interacting protein 3 Kit ELISA, BCL2/adenovirus E1B interacting protein 3-like Kit ELISA, BCL2/adenovirus E1B interacting protein 3-like b Kit ELISA, BCL2/adenovirus E1B interacting protein 3-like a Kit ELISA, BNIP3L Kit ELISA, BNIP3 Kit ELISA, Bnip3l Kit ELISA, Bnip3 Kit ELISA, bnip3lb Kit ELISA, bnip3la Kit ELISA

ID gène: 665

UniProt: O60238

Pathways: Autophagy