SULT1A1 Kit ELISA

N° du produit ABIN6951477

Détail du produit SULT1A1 Kit ELISA

Antigène: SULT1A1 (Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human SULT1A1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: SULT1A1 (Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1))

Autre désignation: SULT1A1 (SULT1A1 Produits)

Synonymes: HAST1/HAST2 Kit ELISA, P-PST Kit ELISA, PST Kit ELISA, ST1A1 Kit ELISA, ST1A3 Kit ELISA, STP Kit ELISA, STP1 Kit ELISA, TSPST1 Kit ELISA, AI266890 Kit ELISA, Stp Kit ELISA, Stp1 Kit ELISA, ASTIV Kit ELISA, Mx-ST Kit ELISA, PST-1 Kit ELISA, St1a1 Kit ELISA, Stm Kit ELISA, Sult1a3 Kit ELISA, pst Kit ELISA, st1a3 Kit ELISA, stp Kit ELISA, stp1 Kit ELISA, tspst1 Kit ELISA, cSULT1A1 Kit ELISA, SULT1A2 Kit ELISA, SULT1A1 Kit ELISA, SIAT8-D Kit ELISA, ST8SiaIV Kit ELISA, Siat8d Kit ELISA, PST1 Kit ELISA, SIAT8D Kit ELISA, ST8SIA-IV Kit ELISA, sulfotransferase family 1A member 1 Kit ELISA, sulfotransferase family 1A, phenol-preferring, member 1 Kit ELISA, sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1 Kit ELISA, sulfotransferase family 1A member 1 S homeolog Kit ELISA, sulfotransferase 1A1 Kit ELISA, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 Kit ELISA, SULT1A1 Kit ELISA, Sult1a1 Kit ELISA, sult1a1 Kit ELISA, sult1a1.S Kit ELISA, LOC704658 Kit ELISA, LOC709318 Kit ELISA, St8sia4 Kit ELISA, ST8SIA4 Kit ELISA, LOC100064221 Kit ELISA

ID gène: 6817

UniProt: P50225