ATG5 Kit ELISA

N° du produit ABIN6951530

Détail du produit ATG5 Kit ELISA

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ATG5 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Autre désignation: ATG5 (ATG5 Produits)

Synonymes: APG5 Kit ELISA, APG5-LIKE Kit ELISA, APG5L Kit ELISA, ASP Kit ELISA, hAPG5 Kit ELISA, ATG5 Kit ELISA, CG1643 Kit ELISA, DmAtg5 Kit ELISA, Dmel\\CG1643 Kit ELISA, atg5 Kit ELISA, 2010107M05Rik Kit ELISA, 3110067M24Rik Kit ELISA, AW319544 Kit ELISA, Apg5l Kit ELISA, Atg5l Kit ELISA, C88337 Kit ELISA, Paddy Kit ELISA, apg5l Kit ELISA, zgc:100934 Kit ELISA, ATATG5 Kit ELISA, AUTOPHAGY 5 Kit ELISA, MKP11.20 Kit ELISA, MKP11_20 Kit ELISA, autophagy related 5 Kit ELISA, Autophagy-related 5 Kit ELISA, ATG5 autophagy related 5 homolog (S. cerevisiae) Kit ELISA, autophagy related 5 L homeolog Kit ELISA, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 Kit ELISA, autophagy protein Apg5 family Kit ELISA, ATG5 Kit ELISA, Atg5 Kit ELISA, atg5 Kit ELISA, atg5.L Kit ELISA, APG5 Kit ELISA

ID gène: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy