Neuroserpin Kit ELISA

N° du produit ABIN6951558

Détail du produit Neuroserpin Kit ELISA

Antigène: Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human SerpinI1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))

Autre désignation: SerpinI1 (SERPINI1 Produits)

Synonymes: PI12 Kit ELISA, neuroserpin Kit ELISA, AI837402 Kit ELISA, Ns Kit ELISA, PI-12 Kit ELISA, Spi17 Kit ELISA, CG9453 Kit ELISA, Dmel\\CG9453 Kit ELISA, Serp2 Kit ELISA, Sp4 Kit ELISA, Spn4 Kit ELISA, Spn4A Kit ELISA, dSerp2 Kit ELISA, sp4 Kit ELISA, spn4 Kit ELISA, raPIT5a Kit ELISA, SERPINI1 Kit ELISA, pi12 Kit ELISA, serpini1l Kit ELISA, si:ch211-167c22.4 Kit ELISA, serpin family I member 1 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade I, member 1 Kit ELISA, Serpin 42Da Kit ELISA, serpin peptidase inhibitor, clade I (neuroserpin), member 1 Kit ELISA, serpin family I member 1 L homeolog Kit ELISA, SERPINI1 Kit ELISA, Serpini1 Kit ELISA, Spn42Da Kit ELISA, serpini1 Kit ELISA, serpini1.L Kit ELISA

ID gène: 5274

UniProt: Q99574

Pathways: Regulation of Hormone Metabolic Process