IL-1 beta Kit ELISA

N° du produit ABIN6963660

Le kit ELISA IL-1 beta (ABIN6963660) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat IL-1 beta. Ce kit ELISA a été cité dans 62 publications.

Aperçu rapide pour IL-1 beta Kit ELISA (ABIN6963660)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL-1 beta Kit ELISA

Seuil minimal de détection: 31.25 pg/mL

Fonction: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Analytical Method: Quantitative

Specificité: This kit recognizes Rat IL-1β in samples. No significant cross-reactivity or interference between Rat IL-1β and analogues was observed

Sensibilité: 18.75 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Préparation des réactifs:

1. Equilibrate all reagents to room temperature (18-25 °C) before use. If the kit will not be used up in one assay, please only take out the necessary strips and reagents for present experiment, and store the remaining strips and reagents at required condition.
2. Standard Working Solution: Centrifuge the standard at 10,000x g for 1 min. Reconstitute with 1 mL of the Standard & Sample Diluent, allow to stand for 10 minutes, and gently invert several times until completely dissolved. Alternatively, vortex briefly at low speed, remove bubbles by low-speed centrifugation if necessary.This produces a 2000 pg/mL stock solution. Prepare serial dilutions according to experimental requirements. Recommended dilution gradient: 2000, 1000, 500, 250, 125, 62.5, 31.25, 0 pg/mL Serial Dilution Procedure: Dispense 500 μL of Standard & Sample Diluent into 7 EP tubes, respectively. Transfer 500 μL of the stock solution into the first tube. Sequentially transfer 500 μL from the preceding tube into the next tube for subsequent dilutions. Designate the last tube as the blank, do not transfer any solution into it. Aliquot the stock and store at -20 ° C. Use within two weeks and avoid repeated freeze-thaw cycles. Serially diluted standards should be prepared immediately, prior to use.
3. Biotinylated Detection Ab working solution: Calculate the required volume (100 μL/well) plus a slight excess. Centrifuge the Concentrated Biotinylated Detection Antibody (100x) at 800 x g for 1 minute. Dilute to 1x with Biotinylated Detection Ab Diluent at a ratio of 1:99. Prepare the working solution immediately, prior to use.
4. HRP Conjugate Working Solution: Calculate the required volume (100 μL/well) plus a slight excess. Centrifuge the Concentrated HRP Conjugate (100x) at 800 x g for 1 minute. Centrifuge the Concentrated HRP Conjugate (100x) at 800 x g for 1 minute. Prepare the working solution immediately, prior to use.
5. Wash Buffer: Prepare the working Wash Buffer by diluting 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water yielding 750 mL. Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved. For same day use only.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-1β were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-1β were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Date de péremption: 12 months

Références pour IL-1 beta Kit ELISA (ABIN6963660)

Adedara, Fabunmi, Ayenitaju, Atanda, Adebowale, Ajayi, Owoeye, Rocha, Farombi: "Neuroprotective mechanisms of selenium against arsenic-induced behavioral impairments in rats." dans: Neurotoxicology, Vol. 76, pp. 99-110, (2021) (PubMed).

Chen, Zhang, Li, Liu, Wang, Zhang, Xu, Li, Guo, Yao: "Berberine regulates fecal metabolites to ameliorate 5-fluorouracil induced intestinal mucositis through modulating gut microbiota." dans: Biomedicine & pharmacotherapy, Vol. 124, pp. 109829, (2021) (PubMed).

Han, Chen, Chen, Luo, Liu, He, He, Qiu, Chen, Wu, Zhuang, Yang, Wu, Wu, Yang, Ge, Zhuang, Yu: "Tetramethylpyrazine attenuates endotoxin-induced retinal inflammation by inhibiting microglial activation via the TLR4/NF-κB signalling pathway." dans: Biomedicine & pharmacotherapy, Vol. 128, pp. 110273, (2021) (PubMed).

Yang, Gao, Han, Li, Liao, Li, Wang, Luo: "Hippocampal changes in inflammasomes, apoptosis, and MEMRI after radiation-induced brain injury in juvenile rats." dans: Radiation oncology (London, England), Vol. 15, Issue 1, pp. 78, (2021) (PubMed).

Albrakati: "Aged garlic extract rescues ethephon-induced kidney damage by modulating oxidative stress, apoptosis, inflammation, and histopathological changes in rats." dans: Environmental science and pollution research international, Vol. 28, Issue 6, pp. 6818-6829, (2021) (PubMed).

Ling, Zhou, Guo, Hu, Chu, Xie, Li, Wang: "H2S attenuates oxidative stress via Nrf2/NF-κB signaling to regulate restenosis after percutaneous transluminal angioplasty." dans: Experimental biology and medicine (Maywood, N.J.), Vol. 246, Issue 2, pp. 226-239, (2021) (PubMed).

Yang, Jia, Li, Mehmood: "Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats." dans: Experimental biology and medicine (Maywood, N.J.), Vol. 245, Issue 14, pp. 1280-1289, (2021) (PubMed).

Song, Sun, Li, Long, Yan, Qin, Makinde, Famurewa, Jaisi, Nie, Olatunji: "Tiliacora triandra extract and its major constituent attenuates diabetic kidney and testicular impairment by modulating redox imbalance and pro-inflammatory responses in rats." dans: Journal of the science of food and agriculture, Vol. 101, Issue 4, pp. 1598-1608, (2021) (PubMed).

Faheem, Rehman, Jabeen, Akash: "Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance." dans: Environmental toxicology and pharmacology, Vol. 80, pp. 103506, (2021) (PubMed).

Hu, Guo, Dai, Wang, Gong, Yu, Peng, Li: "Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-κB and TGF-β/smads signaling pathways." dans: Journal of ethnopharmacology, Vol. 262, pp. 113275, (2021) (PubMed).

Lin, Wang, Lu, Huang, Panyod, Liu, Yang, Chen, Lu, Sheen: "Water extract of Armillaria mellea (Vahl) P. Kumm. Alleviates the depression-like behaviors in acute- and chronic mild stress-induced rodent models via anti-inflammatory action." dans: Journal of ethnopharmacology, Vol. 265, pp. 113395, (2021) (PubMed).

Owumi, Adedara, Farombi, Oyelere: "Protocatechuic acid modulates reproductive dysfunction linked to furan exposure in rats." dans: Toxicology, Vol. 442, pp. 152556, (2021) (PubMed).

Liu, He, Wang, Wang, Sun, Li, Jia, Wang, Xu, Ming, Wang, Lin: "Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways." dans: Journal of ethnopharmacology, Vol. 260, pp. 113039, (2021) (PubMed).

Eraslan, Tanyeli, Polat, Yetim: "Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study." dans: Journal of cellular biochemistry, Vol. 120, Issue 10, pp. 17159-17166, (2020) (PubMed).

Ge, Sun, Fei, Gao, Pan, Wu: "Hydrogen sulfide treatment alleviated ventilator-induced lung injury through regulation of autophagy and endoplasmic reticulum stress." dans: International journal of biological sciences, Vol. 15, Issue 13, pp. 2872-2884, (2020) (PubMed).

Liu, Cao, Zhang, Peng: "Electroacupuncture reduces astrocyte number and oxidative stress in aged rats with surgery-induced cognitive dysfunction." dans: The Journal of international medical research, Vol. 47, Issue 8, pp. 3860-3873, (2020) (PubMed).

Çakır, Tekin, Okan, Çakan, Doğanyiğit: "The ameliorating effect of cannabinoid type 2 receptor activation on brain, lung, liver and heart damage in cecal ligation and puncture-induced sepsis model in rats." dans: International immunopharmacology, Vol. 78, pp. 105978, (2020) (PubMed).

Dong, Zhuang, Ning, Wu, Lu, Wan: "Palmitic acid stimulates NLRP3 inflammasome activation through TLR4-NF-κB signal pathway in hepatic stellate cells." dans: Annals of translational medicine, Vol. 8, Issue 5, pp. 168, (2020) (PubMed).

Giménez-Siurana, Gómez García, Pagan Bernabeu, Lozano-Pérez, Aznar-Cervantes, Cenis, López-Jornet: "Chemoprevention of Experimental Periodontitis in Diabetic Rats with Silk Fibroin Nanoparticles Loaded with Resveratrol." dans: Antioxidants (Basel, Switzerland), Vol. 9, Issue 1, (2020) (PubMed).

Güvenç, Cellat, Uyar, Özkan, Gokcek, İsler, Yakan: "Nobiletin Protects from Renal Ischemia-Reperfusion Injury in Rats by Suppressing Inflammatory Cytokines and Regulating iNOS-eNOS Expressions." dans: Inflammation, Vol. 43, Issue 1, pp. 336-346, (2020) (PubMed).

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: Interleukin 1 Beta

Sujet: Target Synonym: Il1b

UniProt: Q63264

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome