IL-6 Kit ELISA

N° du produit ABIN6973862

Détail du produit IL-6 Kit ELISA

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 pg/mL - 20 pg/mL

Seuil minimal de détection: 0.312 pg/mL

Application: ELISA

Fonction: For the quantitative determination of rat interleukin 6 (IL-6) concentrations in serum, plasma, tissue homogenates.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of rat IL-6. No significant cross-reactivity or interference between rat IL-6 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rat IL-6 and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.078 pg/mL

Ingrédients:

• Assay plate
• Standard
• HRP-avidin (100 x concentrate)
• Biotin-antibody (100 x concentrate)
• Sample Diluent
• HRP-avidin Diluent
• Biotin-antibody Diluent
• Wash Buffer (25 x concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole:

1. Prepare reagents, samples and standards as instructed.
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
3. Remove the liquid of each well, don't wash.
4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
5. Aspirate and wash 3 times.
6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
7. Aspirate and wash 5 times.
8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.

Préparation des réactifs:

1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
3. Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 20 pg/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (20 pg/mL). Sample Diluent serves as the zero standard (0 pg/mL).

Note:

• Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
• Bring all reagents to room temperature (18-25 °C) before use for 30 min.
• Prepare fresh standard for each assay. Use within 4 hours and discard after use.
• Making serial dilution in the wells directly is not permitted.
• Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
• Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Note:

Recommend to dilute the serum or plasma samples with Sample Diluent (1:10) before test. The suggested 10-fold dilution can be achieved by adding 25 μL sample to 225 μL of Sample Diluent. The recommended dilution factor is for reference only. The optimal dilution factor should be determined by users according to their particular experiments.

Précision du teste: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.

Date de péremption: 6 months

Références pour IL-6 Kit ELISA (ABIN6973862)

Nair, Gagnon, Pelletier, Tchoukanova, Zhang, Ewart, Ewart, Jiao, Wang: "Shrimp oil extracted from the shrimp processing waste reduces the development of insulin resistance and metabolic phenotypes in diet-induced obese rats." dans: Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme, Vol. 42, Issue 8, pp. 841-849, (2018) (PubMed).

Adamu, Imam, Der-Jiun, Ismail: "In utero Exposure to Germinated Brown Rice and Its GABA Extract Attenuates High-Fat-Diet-Induced Insulin Resistance in Rat Offspring." dans: Journal of nutrigenetics and nutrigenomics, Vol. 10, Issue 1-2, pp. 19-31, (2018) (PubMed).

Wang, Ding, Tang, Li, Gan: "Effect of Liuweibuqi capsules on the balance between MMP-9 and TIMP1 and viability of alveolar macrophages in COPD." dans: Bioscience reports, Vol. 37, Issue 5, (2018) (PubMed).

Wang, Yi, Du, Wang, Tang, Wang, Qi, Li, Lai, Xia, Tang: "A study of Epstein-Barr virus infection in the Chinese tree shrew(Tupaia belangeri chinensis)." dans: Virology journal, Vol. 14, Issue 1, pp. 193, (2018) (PubMed).

Chen, Zhang, Zhang, He, Zhou, Xie: "Dexmedetomidine reduces the neuronal apoptosis related to cardiopulmonary bypass by inhibiting activation of the JAK2-STAT3 pathway." dans: Drug design, development and therapy, Vol. 11, pp. 2787-2799, (2018) (PubMed).

Nielsen, Roager, Casas, Frandsen, Gosewinkel, Bester, Licht, Hendriksen, Bahl: "Glyphosate has limited short-term effects on commensal bacterial community composition in the gut environment due to sufficient aromatic amino acid levels." dans: Environmental pollution (Barking, Essex : 1987), Vol. 233, pp. 364-376, (2018) (PubMed).

Zhao, Zhang, Ma, Tian, Shen, Zhou: "A combination of quercetin and resveratrol reduces obesity in high-fat diet-fed rats by modulation of gut microbiota." dans: Food & function, Vol. 8, Issue 12, pp. 4644-4656, (2018) (PubMed).

Eltahir, Nazmy: "Esomeprazole ameliorates CCl4 induced liver fibrosis in rats via modulating oxidative stress, inflammatory, fibrogenic and apoptotic markers." dans: Biomedicine & pharmacotherapy, Vol. 97, pp. 1356-1365, (2018) (PubMed).

Abbas, Kamel, El-Sayed: "Dermal anti-oxidant, anti-inflammatory and anti-aging effects of Compritol ATO-based Resveratrol colloidal carriers prepared using mixed surfactants." dans: International journal of pharmaceutics, Vol. 541, Issue 1-2, pp. 37-47, (2018) (PubMed).

Chepurnova, Samoilova, Anisimov, Verin, Korotaeva: "Compounds of IL-6 Receptor Complex during Acute Lung Injury." dans: Bulletin of experimental biology and medicine, Vol. 164, Issue 5, pp. 609-611, (2018) (PubMed).

Li, Li, Liu, Wang, Zhang, Li, Yang, Song, Yin, Gao, Cheng, Cai, Tian: "Telmisartan Ameliorates Nephropathy in Metabolic Syndrome by Reducing Leptin Release From Perirenal Adipose Tissue." dans: Hypertension (Dallas, Tex. : 1979), Vol. 68, Issue 2, pp. 478-90, (2017) (PubMed).

Wang, Cui, Chen, Deng, Liao, Wei, Li, Su, Yu, Yi: "Prenatal Exposure to Lipopolysaccharide Alters Renal DNA Methyltransferase Expression in Rat Offspring." dans: PLoS ONE, Vol. 12, Issue 1, pp. e0169206, (2017) (PubMed).

Xie, Min, Chen, Yang, Wang: "Ulinastatin inhibited sepsis-induced spinal inflammation to alleviate peripheral neuromuscular dysfunction in an experimental rat model of neuromyopathy." dans: Journal of neurochemistry, Vol. 143, Issue 2, pp. 225-235, (2017) (PubMed).

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Tang, Yang, Zhang, Ren, Sang, Lu, Zhong, Mao: "Magnesium isoglycyrrhizinate inhibits inflammatory response through STAT3 pathway to protect remnant liver function." dans: World journal of gastroenterology, Vol. 21, Issue 43, pp. 12370-80, (2016) (PubMed).

Adamu, Imam, Ooi, Esa, Rosli, Ismail: "Perinatal exposure to germinated brown rice and its gamma amino-butyric acid-rich extract prevents high fat diet-induced insulin resistance in first generation rat offspring." dans: Food & nutrition research, Vol. 60, pp. 30209, (2016) (PubMed).

Guo, Ding, Huang, Wang, Meng, Xiao: "Total saponin of Dioscoreae hypoglaucae rhizoma ameliorates streptozotocin-induced diabetic nephropathy." dans: Drug design, development and therapy, Vol. 10, pp. 799-810, (2016) (PubMed).

Abdul Shukkoor, Baharuldin, Mat Jais, Mohamad Moklas, Fakurazi: "Antidepressant-Like Effect of Lipid Extract of Channa striatus in Chronic Unpredictable Mild Stress Model of Depression in Rats." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 2986090, (2016) (PubMed).

Mohammad Reza, Hamideh, Zahra: "The Nociceptive and Anti-Inflammatory Effects of Artemisia dracunculus L. Aqueous Extract on Fructose Fed Male Rats." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 895417, (2015) (PubMed).

Zhang, Li, Jiang, Chen, Huang: "Neuroprotective Effects of (-)-Epigallocatechin-3-Gallate Against Focal Cerebral Ischemia/Reperfusion Injury in Rats Through Attenuation of Inflammation." dans: Neurochemical research, Vol. 40, Issue 8, pp. 1691-8, (2015) (PubMed).

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL6 (IL6 Produits)

Synonymes: BSF2 Kit ELISA, HGF Kit ELISA, HSF Kit ELISA, IFNB2 Kit ELISA, IL-6 Kit ELISA, Il-6 Kit ELISA, ILg6 Kit ELISA, Ifnb2 Kit ELISA, il6 Kit ELISA, CHIL-6 Kit ELISA, interleukin 6 Kit ELISA, interleukin-6 Kit ELISA, IL6 Kit ELISA, Il6 Kit ELISA, il-6 Kit ELISA, IL-6 Kit ELISA

Sujet: IL6

UniProt: P20607

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome