ITGB1 Kit ELISA

N° du produit ABIN7035622

Détail du produit ITGB1 Kit ELISA

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: The kit is an instand sandwich enzyme immunoassay for in vitro quantitative measurement in various sample types.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Integrin Beta 1.

Sensibilité: 0.31 ng/mL

Classe de qualité: Instant

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 100 µL

Durée du test: 1.5 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 30 minutes at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 30 minutes at 37 °C,
4. Aspirate and wash 5 times,
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Préparation des réactifs:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 500ng/mL. Firstly dilute the stock solution to 50 ng/mL and the diluted standard serves as the highest standard (50 ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and produce a double dilution series by transferring 500 µL each. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL, 1.56ng/mL, 0.78ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0ng/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. Prepare Substrate working Solution within 15 minutes before assay.
6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
7. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 ° C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

Date de péremption: 6 months

Détail du antigène

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Autre désignation: Integrin Beta 1 (ITGB1 Produits)

Synonymes: CD29 Kit ELISA, FNRB Kit ELISA, GPIIA Kit ELISA, MDF2 Kit ELISA, MSK12 Kit ELISA, VLA-BETA Kit ELISA, VLAB Kit ELISA, 4633401G24Rik Kit ELISA, AA409975 Kit ELISA, AA960159 Kit ELISA, ENSMUSG00000051907 Kit ELISA, Fnrb Kit ELISA, Gm9863 Kit ELISA, gpIIa Kit ELISA, cd29 Kit ELISA, fnrb Kit ELISA, gpiia Kit ELISA, itgb1 Kit ELISA, mdf2 Kit ELISA, msk12 Kit ELISA, vla-beta Kit ELISA, vlab Kit ELISA, itgb1-a Kit ELISA, itgb1-b Kit ELISA, integrin subunit beta 1 Kit ELISA, integrin beta 1 (fibronectin receptor beta) Kit ELISA, integrin subunit beta 1 S homeolog Kit ELISA, integrin beta 1 Kit ELISA, integrin subunit beta 1 L homeolog Kit ELISA, ITGB1 Kit ELISA, Itgb1 Kit ELISA, itgb1.S Kit ELISA, LOC100009173 Kit ELISA, itgb1.L Kit ELISA

Sujet: CD29, MSK12, ITG-B1, FNRB, GPIIA, MDF2, MSK12, VLAB, Fibronectin Receptor Beta Polypeptide, Antigen CD29 Includes MDF2, Glycoprotein IIa, VLA-4 subunit beta

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome