IL-6 Kit ELISA

N° du produit ABIN7671732

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris IL-6.

Aperçu rapide pour IL-6 Kit ELISA (ABIN7671732)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.563 pg/mL - 100 pg/mL

Application: ELISA

Type d'échantillon: Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Classe de qualité: Quick

Détail du produit IL-6 Kit ELISA

Seuil minimal de détection: 1.563 pg/mL

Fonction: Mouse IL-6 (Interleukin 6) QuickTest ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with IL-6 , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (When the sample content is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Normal mouse serum/plasma (EDTA, Citrate , heparin) (n=16) undiluted ND-42pg/mL Normal mouse were intraperitoneally injected with 50 μg LPS, and the serum was detected 12 hours later (n=3) 1/10-1/50 dilution 862-1886pg/mL Mouse spleen tissue homogenates undiluted 133pg/1 mg(total protein) RAW 264.7 cells were cultured with 5 %FBS + 1640 + double antibody for 7 hours 1/2 dilution 25pg/mL RAW 264.7 cells were cultured with 5 %FBS+ 1640 + double antibody +100 ng/mL LPS, and the cell culture supernatant was detected after 7 hours 1/10 dilution 712pg/mL 2. KD sample validation (Detect IL-6-KD RAW 264.7 cells): Sample Type Dilution Ratio Content Wild RAW 264.7 cells were stimulated with 100 ng/mL LPS for 7 hours to detect cell culture supernatant 1/10 dilution 811pg/mL RAW 264.7 cells (+siRNA) were stimulated with 100 ng/mL LPS for 7 hours to detect cell culture supernatants undiluted ND RAW 264.7 cells (+siRNA) were treated with 100 ng/mL LPS for 7 hours and then cultured with 300 ng/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 1.69 mg/mL) to detect. undiluted 27pg/mg(total protein) Note:ND is lower than the sensitivity of the kit and was not detected 3. Antibody by WB KD validation (Detect IL-6-KO RAW 264.7 cells):

Sensibilité: 0.938 pg/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Cap/Det Ab (Ready to use
• blue)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Sandwich ELISA, Double Antibody

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37 °C. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37 °C. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Procédure de l'essai:

1. Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
2. Wash the plate twice without immersion.
3. Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
4. Wash the plate five times without immersion.
5. Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
6. Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL-6

Sujet: Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2

UniProt: P08505

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome