FAS Kit ELISA

N° du produit ABIN7830644

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain FAS.

Aperçu rapide pour FAS Kit ELISA (ABIN7830644)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit FAS Kit ELISA

Fonction: FAS (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FAS expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: FAS

Sujet:

Synonyms: ALPS1A|APO-1|APT1|CD95|FAS1|FASTM|TNFRSF6

Gene Description: Fas (TNF receptor superfamily, member 6)

ID gène: 355

UniProt: P25445

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity