Insulin Receptor Kit ELISA

N° du produit ABIN7830705

Le kit ELISA Insulin Receptor (ABIN7830705) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Insulin Receptor.

Aperçu rapide pour Insulin Receptor Kit ELISA (ABIN7830705)

Antigène: Insulin Receptor (INSR)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit Insulin Receptor Kit ELISA

Fonction: INSR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of INSR expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: Insulin Receptor (INSR)

Autre désignation: INSR

Sujet:

Synonyms: CD220|HHF5

Gene Description: insulin receptor

ID gène: 3643

UniProt: P06213

Pathways: Signalisation NF-kappaB, Signalisation RTK, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity