JAK3 Kit ELISA

N° du produit ABIN7830714

Le kit ELISA JAK3 (ABIN7830714) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain JAK3.

Aperçu rapide pour JAK3 Kit ELISA (ABIN7830714)

Antigène: JAK3 (Janus Kinase 3 (JAK3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit JAK3 Kit ELISA

Fonction: JAK3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JAK3 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: JAK3 (Janus Kinase 3 (JAK3))

Autre désignation: JAK3

Sujet:

Synonyms: JAK-3|JAK3_HUMAN|JAKL|L-JAK|LJAK

Gene Description: Janus kinase 3 (a protein tyrosine kinase, leukocyte)

ID gène: 3718

UniProt: P52333

Pathways: Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response