PKC epsilon Kit ELISA

N° du produit ABIN7830856

Kit ELISA PKC epsilon Humain, Colorimetric test pour la quantification de Humain PKC epsilon.

Aperçu rapide pour PKC epsilon Kit ELISA (ABIN7830856)

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit PKC epsilon Kit ELISA

Fonction: PRKCE (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKCE expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Autre désignation: PRKCE

Sujet:

Synonyms: MGC125656|MGC125657|PKCE|nPKC-epsilon

Gene Description: protein kinase C, epsilon

ID gène: 5581

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling