IL-6 Kit ELISA

N° du produit ABIN7832831

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain IL-6.

Aperçu rapide pour IL-6 Kit ELISA (ABIN7832831)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Classe de qualité: QuickTest

Détail du produit IL-6 Kit ELISA

Fonction: FastELISA IL-6 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human IL-6 (Interleukin 6) in serum, plasma.

Analytical Method: Quantitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freezing and thawing

Stock: 4 °C

Stockage commentaire: Store at 2-8C for 6 months.Avoid repeated freezing and thawing.

Date de péremption: 6 months

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL-6

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome