GRB2 Kit ELISA

N° du produit ABIN8080240

Kit ELISA GRB2 Souris, Colorimetric test pour la quantification de Souris GRB2.

Aperçu rapide pour GRB2 Kit ELISA (ABIN8080240)

Antigène: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Détail du produit GRB2 Kit ELISA

Seuil minimal de détection: 0.312 ng/mL

Fonction: Mouse Growth Factor Receptor Bound Protein 2 (Grb2) ELISA Kit for detecting Grb2 in tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensibilité: 0.113 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Précision du teste: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C for one month, Long term: see manual.

Date de péremption: 12 months

Détail du antigène

Antigène: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Autre désignation: Growth Factor Receptor Bound Protein 2

Sujet: Gene Aliases: ASH, NCKAP2, EGFRBP-GRB2, Grb3-3, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2

ID gène: 14784

UniProt: Q60631

Pathways: Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation