PKC epsilon Kit CLIA

N° du produit ABIN8085863

Ce kit ELISA Chemiluminescent est destiné à la mesure quantitative de Humain PKC epsilon.

Aperçu rapide pour PKC epsilon Kit CLIA (ABIN8085863)

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 0.14 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Détail du produit PKC epsilon Kit CLIA

Seuil minimal de détection: 0.14 ng/mL

Fonction: Human Protein Kinase C Epsilon (PKCe) CLIA Kit for detecting PKCe in tissue homogenates

Analytical Method: Quantitative

Sensibilité: 0.07 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Précision du teste: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C for one month, Long term: see manual.

Date de péremption: 12 months

Détail du antigène

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Autre désignation: Protein Kinase C Epsilon

Sujet: Gene Aliases: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon

ID gène: 5581

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling