PDK1 Kit ELISA

N° du produit ABIN8086053

Le kit ELISA PDK1 (ABIN8086053) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat PDK1.

Aperçu rapide pour PDK1 Kit ELISA (ABIN8086053)

Antigène: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Détail du produit PDK1 Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit for detecting PDK1 in tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensibilité: 0.057 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Précision du teste: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C for one month, Long term: see manual.

Date de péremption: 12 months

Détail du antigène

Antigène: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Autre désignation: Pyruvate Dehydrogenase Kinase Isozyme 1

Sujet: Gene Aliases: [Pyruvate dehydrogenase (acetyl-transferring) ] kinase isozyme 1, mitochondrial

UniProt: Q63065

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Regulation of Carbohydrate Metabolic Process, Hepatitis C, L'effet Warburg