IL-6 Kit ELISA

N° du produit ABIN8090743

Kit ELISA IL-6 Humain, Colorimetric test pour la quantification de Humain IL-6.

Aperçu rapide pour IL-6 Kit ELISA (ABIN8090743)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.078 pg/mL - 5 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Classe de qualité: High Sensitivity

Détail du produit IL-6 Kit ELISA

Seuil minimal de détection: 0.078 pg/mL

Fonction: Human HS-IL-6 (High sensitive Interleukin 6) high sensitive Accquant ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with IL-6 , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (The matrix components in serum/plasma will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing! When the content of other samples is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy human serum/plasma (EDTA, Citrate , heparin) (n=10) 1/2 dilution 1.2-6pg/mL Antigen-positive serum of COVID-19 patients (n=5) 1/300- 1/1000 dilution 132-610pg/mL Antigen-positive plasma (EDTA) of COVID-19 patients (n=12) 1/300-1/2000 dilution 185-996pg/mL Healthy human saliva (n=9) 1/2 dilution ND-12pg/mL Human peripheral blood mononuclear cells (2 x 10^6 cells/mL) were cultured with 5 %FBS+ 1640 + double antibody +10 μg PHA/mL, and the cell culture supernatant was detected after 48 hours 1/10000 dilution 5.2 ng/mL 2. KO sample validation (Detect IL-6 KO A549 cells): Sample Type Dilution Ratio Content Wild A549 cells were stimulated with 20 ng/mL(h.IL-11b) for 24 hours to detect cell culture supernatant 1/3000 dilution 1.56 ng/mL KO A549 cells were stimulated with 20 ng/mL(h.IL-11b) for 24 hours to detect cell culture supernatants 1/2 dilution ND KO A549 cells were treated with 20 ng/mL(H.IL-11b) for 24 hours and then cultured with 5 μg/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 2.51 mg/mL) to detect. 1/2 dilution ND Note:ND is lower than the sensitivity of the kit and was not detected 3. Capture antibody and detection antibody by WB KO validation (Detect IL-6 KO A549 cells):

Sensibilité: 0.046 pg/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Sandwich ELISA, Double Antibody

Durée du test: 5 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL-6

Sujet: Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2

UniProt: P05231

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome