IL-6 Kit ELISA
Aperçu rapide pour IL-6 Kit ELISA (ABIN8126252)
Antigène
Voir toutes IL-6 (IL6) Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 15.6 pg/mL
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Fonction
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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Sensibilité
- 9.375 pg/mL
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Attributs du produit
- This immunoassay kit allows for the in vitro quantitative determination of mouse IL-6 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.375 pg/mL
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Interleukin 6 (IL6) ELISA Kit
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Plaque
- Pre-coated
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Protocole
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader, and then the concentration of IL-6 can be calculated.
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Restrictions
- For Research Use only
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Stockage commentaire
- Please refer to the Manual
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- IL-6 (IL6) (Interleukin 6 (IL6))
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Autre désignation
- IL-6 (Interleukin 6)
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Pathways
- Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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