IL12 Kit ELISA

N° du produit ABIN8126256

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Chien IL12.

Aperçu rapide pour IL12 Kit ELISA (ABIN8126256)

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Chien

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL12 Kit ELISA

Seuil minimal de détection: 15.625 pg/mL

Fonction: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensibilité: 9.375 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Canine IL-12/p70 concentrations in serum, plasma. Detection Range: 15.625 - 1000 pg/mL Sensitivity: < 9.375 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-canine IL-12/p70 antibody was pre-coated into 96-well plates. Biotin conjugated anti-canine IL-12/p70 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the canine IL-12/p70 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Canine IL-12/p70 can be calculated using the standard curve.

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12/p70 (Interleukin 12/p70)

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome