IL-1 beta Kit ELISA
-
- Antigène Voir toutes IL-1 beta (IL1B) Kits ELISA
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
-
Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6-1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Fonction
- This ELISA kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of rat IL1B in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of rat IL1B. No significant cross- reactivity or interference was observed.
- Sensibilité
-
The minimum detectable dose of rat IL1 B is typically less than 4.2 pg/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by the mean O.D. value of 20 replicates of the zero calibrator plus three standard deviations. - Attributs du produit
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL1B. Calibrators and samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the TMB substrate solution is added to each well. Only those wells that contain IL1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL1B in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- Ingrédients
-
Pre-coated, ready to use 96-well plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - Matériel non inclus
-
1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
- Discover our best selling IL1B Kit ELISA
- Top Product
- Discover our top product IL1B Kit ELISA
-
-
- Commentaires
-
The calibration curve concentrations used for the ELISA's were 1000, 500, 250, 125, 62.5, 31.2, 15.6 pg/mL.
- Plaque
- Pre-coated
- Calcul des résultats
-
Average the duplicate readings for each calibrator, control, and sample and subtract the average zero calibrator optical density. Create a calibration curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a calibration curve by plotting the mean absorbance for each calibrator on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL1B concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. It is recommended to use some related software to do this calculation. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- Restrictions
- For Research Use only
-
- Stock
- -20 °C
- Stockage commentaire
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- Date de péremption
- The expiry date is stated on the label.
-
- Antigène Voir toutes IL-1 beta (IL1B) Kits ELISA
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- Autre désignation
- IL1b (IL1B Produits)
- Synonymes
- IL-1 Kit ELISA, IL1-BETA Kit ELISA, IL1F2 Kit ELISA, IL-1BETA Kit ELISA, IL1beta Kit ELISA, il1-b Kit ELISA, zgc:111873 Kit ELISA, IL-1B Kit ELISA, IL-1beta Kit ELISA, Il-1b Kit ELISA, IL1B Kit ELISA, IL-1 beta Kit ELISA, IL-1b Kit ELISA, interleukin 1 beta Kit ELISA, interleukin 1, beta Kit ELISA, IL1B Kit ELISA, il1b Kit ELISA, Il1b Kit ELISA
- Pathways
- Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
-