Anti-Chlamydia Trachomatis Antibody (IgG) Kit ELISA

N° du produit ABIN996990

Détail du produit

Antigène: Anti-Chlamydia Trachomatis Antibody (IgG)

Reactivité: Chlamydia trachomatis

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Fonction: The ELISA, Chlamydia Trachomatis IgG is intended for use in evaluating a patient's serologic status to Chlamydia Trachomatis infection. It is also used to evaluate paired sera for the presence of a significant increase in specific IgG as indicative of a recent or current Chlamydia Trachomatis infection.

Analytical Method: Qualitative

Specificité: 96.1 %

Information d'application

Commentaires:

Quality Control:
The test run may be considered valid provided the following criteria are met: Diagnostic Automation/Cortez Diagnostics, Inc. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 USA Phone: 618.591.3030 Fax 818.591.8383 E m a i I: n e ste p @ r a p i dte st. co m Webs ite : www.rapi dte st. co m
1. The O.D. value of the reagent blank against air from a microwell reader should be less than 0.150.
2. If the O.D. value of the Calibrator is lower than 0.250, the test is not valid and must be repeated.
3. The IgG Index for Negative and Positive Control should be in the range stated on the labels.
Limitations of procedure:
1. A single serum sample cannot be used to determine recent infection.
2. A serum specimen taken in an early stage during acute phase of infection may contain low levels of IgG antibody and render an IgG Index result negative.
3. As with other serological assays, the results of these assays should be used in conjunction with information available from clinical evaluation and other diagnostic procedures.

Plaque: Pre-coated

Procédure de l'essai:

1. Place the desired number of coated strips into the holder.
   2. Prepare 1:40 dilutions by adding 5 μL of the test samples, negative control, positive control, and calibrator to 200 μL of sample diluent. Mix well.
   3. Dispense 100 μL of diluted sera, calibrator, and controls into the appropriate wells. For the reagent blank, dispense 100 μL sample diluent in 1A well position. Tap the holder to remove air bubbles from the liquid and mix well. Incubate for 30 minutes at room temperature.
   4. Remove liquid from all wells. Repeat washing three times with washing buffer.
   5. Dispense 100 μL of enzyme conjugate to each well and incubate for 30 minutes at room temperature.
   6. Remove enzyme conjugate from all wells. Repeat washing three times with washing buffer.
   7. Dispense 100 μL of TMB Chromogenic Substrate to each well and incubate for 15 minutes at room temperature.
   8. Add 100 μL of Stop solution to stop reaction. Make sure there are no air bubbles in each well before reading
   9. Read O.D. at 450 nm with a microwell reader.

Calcul des résultats:

1. To obtain cut off OD Value: Multiply the OD of calibrator by factor (f) printed on label calibrator.
   
   2. Calculate the Chlamydia IgG Index of each determination by dividing the OD value of each sample by obtained OD value of Cut-off. For Example: If Factor (f) value on label = 0.4 Obtained Calibrator O.D =
   1.100 Cut off O.D =
   1.100 X 0.4 = 0.44 (by definition Chlamydia IgG Index = 1) Patient sample O.D = 0.580 Chlamydia IgG Index = 0.580/0.44 =
   1.32 (Positive Result) Patient Sample O.D = 0.320 Chlamydia IgG Index = 0.320 / 0/44 = 0.73 (Negative Result)
   Negative: IgG Index of 0.90 or less are seronegative for IgG antibody. Equivocal: IgG Index of 0.91 - 0.99 are equivocal. Sample should be retested. Positive: IgG Index of
   1.00 or greater. Expected Values: 230 random samples were determined with DIAGNOSTIC AUTOMATION microwell ELISA. Chlamydia Trachomatis IgG. The test results were computed as IgG Index using a chosen reference serum (cut off) as IgG index
   1. The distribution of frequency versus IgG Index is presented as the following: 29 were found to be positive (1
   2.6 %), and 196 were found to be negative (85.2 %).Histogram of Chlamydia T. IgG Index Total samples n=230
   

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C

Détail du antigène

Antigène: Anti-Chlamydia Trachomatis Antibody (IgG)

Classe de substances: Antibody

Sujet: Chlamydia Trachomatis is one of the most common human pathogens. Of the 15 recognized serotypes, 4 (A, B, Ba, and C) have been shown to cause hyperendemic blinding trachoma, a disease which afflicts hundreds of millions of people in developing countries. Three serotypes (L-1, L-2, and L-3) are the causes of lymphogranuloma venereum (LGV), a sexually transmitted systemic disease. The other serotypes (D through K) have been associated with genital tract infections and sporadic cases of conjunctivitis in industrialized societies. These agents are the major recognized cause of nongonococcal urethritis in men, in whom they may also cause epididymitis. In women, C. trachomatis causes cervicitis and has been associated with acute salpingitis. Infants born through an infected birth canal may contract the infection and then develop inclusion conjunctivitis of the newborn and/or the characteristic chlamydial pneumonia syndrome. High levels of anti-Chlamydia IgG antibody are of diagnostic value in chronic or systemic infections such as salpingitis, mechanical infertility, perihepatitis, epididymitis, Reiter’s syndrome and pneumonitis.