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ADA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

ADA WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
N° du produit ABIN1339568
  • Antigène Tous les produits ADA
    ADA (Adenosine Deaminase (ADA))
    Espèce de la protéine
    Humain
    Espèce du lysat
    Human Cells
    Application
    Western Blotting (WB), Immunoprecipitation (IP)
    Fonction
    Transient Overexpression Lysate
    Specificité
    Non-denatured
    Attributs du produit
    ADA HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
    Used Plasmid: pCMV-ADA full length
    Lysed Cells
    HEK 293T Cells
    Lysate Type
    Overexpression Lysate
    Lysate Fraction
    Whole Cell Lysate
  • Indications d'application
    Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    Commentaires

    Product Quality tested by: Transient overexpression cell lysate was tested with Anti-ADA antibody by Western Blots.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2 mg/mL
    Buffer
    Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
    Conseil sur la manipulation
    Aliquot to avoid repeated freezing and thawing.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
  • Antigène
    ADA (Adenosine Deaminase (ADA))
    Autre désignation
    ADA (ADA Produits)
    Synonymes
    ADA-like Lysate, xada Lysate, ADA Lysate, CG11994 Lysate, Dmel\\CG11994 Lysate, DrosADA Lysate, dADA Lysate, zgc:92028 Lysate, adenosine deaminase Lysate, adenosine deaminase S homeolog Lysate, Adenosine deaminase Lysate, ADA Lysate, Ada Lysate, ada.S Lysate, ada Lysate
    Sujet
    Full Gene Name: adenosine deaminase
    Synonyms:
    ID gène
    100
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