A synthetic peptide for use as a blocking control in assays to test for specificity of MRPL45 antibody, Alternative Names: MRPL45 control peptide, MRPL45 antibody Blocking Peptide, Anti-MRPL45 Blocking Peptide, mitochondrial ribosomal protein L45 Blocking Peptide, MGC11321 Blocking Peptide, MRPL45, MRPL-45, MRPL 45, MRPL-45 Blocking Peptide, MRPL 45 Blocking Peptide
Purification
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Optimal conditions should be determined by the investigator
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 µL of distilled water for a final peptide concentration is 1 mg/mL.
Buffer
PBS
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
-20 °C
Stockage commentaire
Store at -20 °C long term.
Antigène
MRPL45
(Mitochondrial Ribosomal Protein L45 (MRPL45))
Synonymes
L45mt Peptide, MRP-L45 Peptide, 2600005P05Rik Peptide, AU043542 Peptide, R75241 Peptide, mrlp45 Peptide, mitochondrial ribosomal protein L45 Peptide, mitochondrial ribosomal protein L45 L homeolog Peptide, Mrpl45 Peptide, MRPL45 Peptide, mrpl45.L Peptide
Sujet
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q.