HNRNPK Protein (AA 3-459, partial) (GST tag)

N° du produit ABIN1047168

Détail du produit

Antigène: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Type de proteíne: Recombinant

Attributs du protein: AA 3-459, partial

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette HNRNPK protéine est marqué à la GST tag.

Application: ELISA

Séquence: TEQPEETFPN TETNGEFGKR PAEDMEEEQA FKRSRNTDEM VELRILLQSK NAGAVIGKGG KNIKALRTDY NASVSVPDSS GPERILSISA DIETIGEILK KIIPTLEEGL QLPSPTATSQ LPLESDAVEC LNYQHYKGSD FDCELRLLIH QSLAGGIIGV KGAKIKELRE NTQTTIKLFQ ECCPHSTDRV VLIGGKPDRV VECIKIILDL ISESPIKGRA QPYDPNFYDE TYDYGGFTMM FDDRRGRPVG FPMRGRGGFD RMPPGRGGRP MPPSRRDYDD MSPRRGPPPP PPGRGGRGGS RARNLPLPPP PPPRGGDLMA YDRRGRPGDR YDGMVGFSAD ETWDSAIDTW SPSEWQMAYE PQGGSGYDYS YAGGRGSYGD LGGPIITTQV TIPKDLAGSI IGKGGQRIKQ IRHESGASIK IDEPLEGSED RIITITGTQD QIQNAQYLLQ NSVKQYADVE GF

Attributs du produit: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Pureté: 90 %

Information d'application

Commentaires:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Conseil sur la manipulation: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Stock: -20 °C

Stockage commentaire: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Référence

Dejgaard, Leffers, Rasmussen, Madsen, Kruse, Gesser, Nielsen, Celis: "Identification, molecular cloning, expression and chromosome mapping of a family of transformation upregulated hnRNP-K proteins derived by alternative splicing." dans: Journal of molecular biology, Vol. 236, Issue 1, pp. 33-48, (1994) (PubMed).

Matunis, Michael, Dreyfuss: "Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein." dans: Molecular and cellular biology, Vol. 12, Issue 1, pp. 164-71, (1992) (PubMed).

Détail du antigène

Antigène: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Autre désignation: Heterogeneous nuclear ribonucleoprotein K protein (HNRNPK Produits)

Synonymes: CSBP Protein, HNRPK Protein, TUNP Protein, Csbp Protein, Hnrpk Protein, hnrpk Protein, MGC75642 Protein, wu:fb37h02 Protein, wu:fi34c04 Protein, zgc:66162 Protein, KBBP Protein, NOVA Protein, heterogeneous nuclear ribonucleoprotein K Protein, heterogeneous nuclear ribonucleoprotein K S homeolog Protein, heterogeneous nuclear ribonucleoprotein k Protein, HNRNPK Protein, Hnrnpk Protein, hnrnpk.S Protein, hnrnpk Protein, LOC5565718 Protein, CpipJ_CPIJ000130 Protein, NGK_p0004 Protein

Sujet: One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.

Poids moléculaire: 78.1 kD

UniProt: P61978