IFI30 Protein (His tag)

N° du produit ABIN1098216

Détail du produit

Antigène: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IFI30 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Pureté: > 90 % by SDS - PAGE

Information d'application

Commentaires:

Synonyms: Gamma-interferon-inducible lysosomal thiol reductase, GILT, IFI-30, IP30

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT

Stock: 4 °C

Stockage commentaire: Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Autre désignation: IFI30 (IFI30 Produits)

Synonymes: GILT Protein, wu:fb12b04 Protein, zgc:101752 Protein, IP30 Protein, IFI-30 Protein, interferon, gamma-inducible protein 30 Protein, IFI30, lysosomal thiol reductase Protein, interferon gamma inducible protein 30 Protein, ifi30 Protein, IFI30 Protein, LOC100304719 Protein, Ifi30 Protein

Sujet: IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Poids moléculaire: 22.5 kDa (199aa) confirmed by MALDI-TOF

NCBI Accession: NP_006323