ALAD Protein (His tag)
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- Antigène Voir toutes ALAD Protéines
- ALAD (Aminolevulinate Dehydratase (ALAD))
- Type de proteíne
- Recombinant
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette ALAD protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Pureté
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product ALAD Protéine
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Aminolevulinate Dehydratase (ALAD) (AA 1-354) protein (His tag)
ALAD Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Aminolevulinate Dehydratase (ALAD) (AA 1-339) protein (GST tag)ALAD Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Aminolevulinate Dehydratase (ALAD) (AA 241-338) protein (GST tag)ALAD Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Aminolevulinate Dehydratase (ALAD) (AA 1-330) protein (Strep Tag)Crystallography grade ALAD Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Aminolevulinate Dehydratase (ALAD) (AA 1-330) protein (Strep Tag)Crystallography grade ALAD Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Aminolevulinate Dehydratase (ALAD) protein (Myc-DYKDDDDK Tag)ALAD Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Commentaires
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Synonyms: Delta-aminolevulinic acid dehydratase, ALADH, PBGS
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1 M NaCl
- Stock
- 4 °C
- Stockage commentaire
- Avoid repeated freezing and thawing cycles.
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- Antigène
- ALAD (Aminolevulinate Dehydratase (ALAD))
- Autre désignation
- ALAD (ALAD Produits)
- Synonymes
- DDBDRAFT_0190269 Protein, DDBDRAFT_0231415 Protein, DDB_0190269 Protein, DDB_0231415 Protein, ALAD Protein, ncf Protein, ALADH Protein, PBGS Protein, ALADR Protein, aminolevulinatedelta-dehydratase Protein, zgc:110219 Protein, Lv Protein, delta-aminolevulinate dehydratase Protein, aminolevulinate dehydratase Protein, delta-aminolevulinic acid dehydratase Protein, Delta-aminolevulinic acid dehydratase Protein, aminolevulinate dehydratase L homeolog Protein, aminolevulinate, delta-, dehydratase Protein, hemB Protein, ALAD Protein, PBGS Protein, STY0404 Protein, SAS1597 Protein, SACI_RS03725 Protein, PAAG_00299 Protein, VDBG_00315 Protein, HPPC_00830 Protein, MGYG_01952 Protein, hem2 Protein, alad.L Protein, Alad Protein, alad Protein
- Sujet
- The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Recombinant human ALAD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 38.8 kDa (354aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_000022
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