TFB2M Protein (His tag)

N° du produit ABIN7279991

Détail du produit

Antigène: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette TFB2M protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Pureté: > 90 % by SDS - PAGE

Information d'application

Commentaires:

Synonyms: Dimethyladenosine transferase 2, mitochondrial, Hkp1, mtTFB2

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol,1 MM DTT

Stock: 4 °C

Stockage commentaire: Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Autre désignation: TFB2M (TFB2M Produits)

Synonymes: CG3910 Protein, Dmel\\CG3910 Protein, TFB2 Protein, d-TF2BM Protein, d-mtTFB2 Protein, TFB2M Protein, sb:cb857 Protein, hkp1 Protein, Hkp1 Protein, mtTFB2 Protein, mitochondrial transcription factor B2 Protein, transcription factor B2, mitochondrial Protein, mtTFB2 Protein, TFB2M Protein, tfb2m Protein, Tfb2m Protein

Sujet: TFB2M is S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. This protein is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. TFB2M stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. Recombinant human TFB2M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 45.8 kDa (401aa) confirmed by MALDI-TOF

NCBI Accession: NP_071761