POLR2J2 Protein (His tag)
-
- Antigène Voir toutes POLR2J2 Protéines
- POLR2J2 (Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2))
- Type de proteíne
- Recombinant
- Origine
- Humain
-
Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette POLR2J2 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Pureté
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product POLR2J2 Protéine
-
-
- Commentaires
-
Synonyms: DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, RPB11b1
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT, 250 mM Imidazole
- Stock
- 4 °C
- Stockage commentaire
- Avoid repeated freezing and thawing cycles.
-
- Antigène
- POLR2J2 (Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2))
- Autre désignation
- POLR2J2 (POLR2J2 Produits)
- Synonymes
- HRPB11B Protein, RPB11b1 Protein, RNA polymerase II subunit J2 Protein, POLR2J2 Protein
- Sujet
- POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. Recombinant human POLR2J2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 15.5 kDa (138aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_116581
-