HMGB1 Protein (BoxA)

N° du produit ABIN1176814

Protéine Recombinant HMGB1 exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour HMGB1 Protein (BoxA) (ABIN1176814)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain, Souris

Source: Escherichia coli (E. coli)

Application: Activation (Act), Cellular Assay (CA)

Pureté: > 98 % It contains no nucleic acids.

Détail du produit

Attributs du protein: BoxA

Fonction: BoxA from HMGB1, human & mouse, LPS-Free

Séquence: BoxA from HMGB1 consists of 89 amino acid: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF

Specificité: BoxA is one of the highly conserved DNA binding domains of HMGB1 protein (HMG boxes), and inhibits HMGB1 effects in vivo.

Attributs du produit: The BoxA we provide has no difference from the sequence of the human or mouse HMGB1, and as a self-antigen elicits very low immunological responses.
BoxA protects against sepsis in a mouse peritonitis model (Yang et al. 2004), and from hepatitis in a mouse model of HBV infection (Sitia et al. 2007).
Dendritic cells promote their own maturation and T cell proliferation, survival and Th1 differentiation by secreting HMGB1, and BoxA inhibits these activities (Dumitriu et al. 2005).
BoxA also inhibits the chemotactic activity of HMGB1 and the mitogenic activity of HMGB1 on stem cells.
BoxA from HMGB1 is produced in E.coli from an expression plasmid coding for the unmodified mammalian sequence, which is totally identical in human and mouse.

Purification: Purified. Oxidation of cysteine 106 makes the protein inactive. To avoid cysteine oxidation DTT 0.5 mM is added during protein purification.

niveau d'endotoxine: LPS free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.

Buffer: The protein is lyophilized from 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Conseil sur la manipulation: The product is resistant to repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C). The product is resistant to repeated freezing and thawing.

Référence

Maroso, Balosso, Ravizza, Liu, Aronica, Iyer, Rossetti, Molteni, Casalgrandi, Manfredi, Bianchi, Vezzani: "Toll-like receptor 4 and high-mobility group box-1 are involved in ictogenesis and can be targeted to reduce seizures." dans: Nature medicine, Vol. 16, Issue 4, pp. 413-9, (2010) (PubMed).

Détail du antigène

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High-mobility group protein B1 (HMGB1)

Sujet: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Poids moléculaire: 10.4 kDa

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome