HMGB1 Protein (BoxB)

N° du produit ABIN1176819

Protéine Recombinant HMGB1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour HMGB1 Protein (BoxB) (ABIN1176819)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain, Souris

Source: Escherichia coli (E. coli)

Application: Activation (Act), Cellular Assay (CA)

Pureté: > 95 % It contains no nucleic acids.

Détail du produit

Attributs du protein: BoxB

Fonction: BoxB from HMGB1, human & mouse, LPS-Free

Séquence: Box B from HMGB1 consists of 90 aminoacid residues: MARIDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAKG KPDAAKKGVV

Attributs du produit: BoxB recapitulates the cytokine activity of full length HMGB1 and efficiently activates macrophages to release tumor necrosis factor (TNF- ) and other proinflammatory cytokines (Li et al 2004).
BoxB from HMGB1 is produced in E.coli from an expression plasmid coding for the mammalian sequence, which is totally identical in human and mouse, preceded by four amino acids (MARI) that have been added for the purpose of expression in bacteria.

Purification: Purified. Oxidation of cysteine 106 makes the protein inactive. To avoid cysteine oxidation DTT 0.5 mM is added during protein purification.

niveau d'endotoxine: LPS free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5mM DTT.

Buffer: The protein is lyophilized from 50 mM HEPES buffer pH 7.9, 500 mM NaCl, 0.5 mM DTT.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Stock: 4 °C/-20 °C

Stockage commentaire: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).

Détail du antigène

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High-mobility group protein B1 (HMGB1)

Sujet: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Poids moléculaire: 10 kDa

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome