HMGB1 Protein (Non-oxidizable)
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- Antigène Voir toutes HMGB1 Protéines
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- Non-oxidizable
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Origine
- Humain, Souris, Rat
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Source
- Escherichia coli (E. coli)
- Application
- Activation (Act), Cellular Assay (CA)
- Séquence
- MGKGDPKKPR GKMSSYAFFV QTSREEHKKK HPDASVNFSE FSKKSSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFSSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE
- Attributs du produit
- This product is a mutant protein where all cysteines are replaced with serines. Non-oxidizable chemokine-HMGB1, LPS free, has chemoattractant activity in vitro and in vivo, does not have cytokine-inducing activity and is resistant to inactivation by ROS. This product is produced in E.coli. The product can be used to recruit leukocytes in vivo without inducing cytokine/chemokine production (Venereau et al, 2012). It contains only trace amounts of LPS (<0.4 ng/mg protein), and is tested for the ability y to induce fibroblast migration. LPS free. Measured by its ability to induce migration. Maximal activity in the cell migration assay is obtained at 1 nM.
- Purification
- Purified
- Pureté
- > 95 %
- niveau d'endotoxine
- The purified protein is free from LPS (Cambrex Limulus Amoebocyte Assay QCL-1000, <0.4 ng LPS per mg protein).
- Top Product
- Discover our top product HMGB1 Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Non-oxidizable chemokine-HMGB1 is lyophilized from 50 mM HEPES buffer, pH 7.9 and 500 mM NaCl.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The protein once resuspended can be stored frozen (-20°C).
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Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release." dans: The Journal of experimental medicine, Vol. 209, Issue 9, pp. 1519-28, (2012) (PubMed).
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Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release." dans: The Journal of experimental medicine, Vol. 209, Issue 9, pp. 1519-28, (2012) (PubMed).
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- Antigène
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Autre désignation
- High-mobility group protein B1 (HMGB1) (HMGB1 Produits)
- Synonymes
- HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein
- Sujet
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HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells. HMGB1 is essential for life: Hmgb1 knockout mice die shortly after birth.HMGB1 contains three conserved cysteine residues (C23, C45 and C106) and is a redox-sensitive. Chemotaxis-HMGB1 (the form with chemoattractanct activity) is completely reduced, cytokine-HMGB1 contains a disulfide bond between C23 and C45, further oxidation to sulfonates abrogate both activities.In Non-oxidizable chemokine-HMGB1 all cysteines are replaced with serines: these replacements preserve chemoattractant activity in vitro and in vivo, eliminate the cytokine-inducing activity and make the protein resistant to inactivation by ROS.
HMGB1 consists of two fairly rigid, L-shaped DNA-binding domains, each referred to as a ‘HMG box’, and an unstructured tail that ends with 30 consecutive negatively charged amino acids. Non-oxidizable chemokine-HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24.8 kDa. - Pathways
- Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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