LC3B Protein (AA 1-125) (GST tag)

N° du produit ABIN1310156

Détail du produit

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Type de proteíne: Recombinant

Attributs du protein: AA 1-125

Origine: Humain

Source: Wheat germ

Purification/Conjugué: Cette LC3B protéine est marqué à la GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Fonction: MAP1LC3B (Human) Recombinant Protein (P01)

Séquence: MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG MKLSV

Attributs du produit: Human MAP1LC3B full-length ORF ( AAH18634, 1 a.a. - 125 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Stockage

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Conseil sur la manipulation: Aliquot to avoid repeated freezing and thawing.

Stock: -80 °C

Stockage commentaire: Best use within three months from the date of receipt of this protein.

Référence

Koukourakis, Giatromanolaki, Bottini, Cappelletti, Zanotti, Allevi, Strina, Ardine, Milani, Brugnoli, Martinotti, Ferrero, Bertoni, Ferrozzi, Harris, Generali: "Prospective neoadjuvant analysis of PET imaging and mechanisms of resistance to Trastuzumab shows role of HIF1 and autophagy." dans: British journal of cancer, Vol. 110, Issue 9, pp. 2209-16, (2014) (PubMed).

Martinet, Schrijvers, Timmermans, Bult, De Meyer: "Immunohistochemical analysis of macroautophagy: recommendations and limitations." dans: Autophagy, Vol. 9, Issue 3, pp. 386-402, (2013) (PubMed).

Koukourakis, Giatromanolaki, Zois, Sivridis: "LC3 immunostaining pitfalls." dans: Histopathology, Vol. 62, Issue 6, pp. 962-3, (2013) (PubMed).

Détail du antigène

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Autre désignation: MAP1LC3B (MAP1LC3B Produits)

Synonymes: ATG8F Protein, LC3B Protein, MAP1A/1BLC3 Protein, MAP1LC3B-a Protein, Map1lc3 Protein, Mpl3 Protein, zbs559 Protein, 1010001C15Rik Protein, Atg8 Protein, LC3b Protein, zgc:56434 Protein, wu:fb60g11 Protein, map1lc3b Protein, MGC76283 Protein, MAP1LC3B Protein, microtubule associated protein 1 light chain 3 beta Protein, microtubule-associated protein 1 light chain 3 beta Protein, microtubule associated protein 1 light chain 3 beta L homeolog Protein, MAP1LC3B Protein, Map1lc3b Protein, map1lc3b Protein, map1lc3b.L Protein

Sujet: Full Gene Name: microtubule-associated protein 1 light chain 3 beta
Synonyms: LC3B,MAP1A/1BLC3

ID gène: 81631

Pathways: Autophagy