CD40 Ligand Protein (CD40LG) (AA 18-111, AA 116-261) (DYKDDDDK Tag)

N° du produit ABIN7676224

Protéine Recombinant CD40 Ligand exprimée dans CHO Cells et a été mentionnée dans 3+ publications.

Aperçu rapide pour CD40 Ligand Protein (CD40LG) (AA 18-111, AA 116-261) (DYKDDDDK Tag) (ABIN7676224)

Antigène: CD40 Ligand (CD40LG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Application: SDS-PAGE (SDS)

Pureté: >95 % (SDS-PAGE)

Détail du produit

Attributs du protein: AA 116-261, AA 18-111

Purification/Conjugué: Cette CD40 Ligand protéine est marqué à la DYKDDDDK Tag.

Fonction: CD40L (human) (multimeric) (rec.)

Specificité: Binds to human CD40.

Réactivité croisée: Humain

Attributs du produit: Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

niveau d'endotoxine: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml). B cell expansion online protocol available.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS

Conseil sur la manipulation: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Stock: 4 °C,-20 °C

Stockage commentaire:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Date de péremption: 6 months

Référence

Kaltenmeier, Gawanbacht, Beyer, Lindner, Trzaska, van der Merwe, Härter, Grüner, Fabricius, Lotfi, Schwarz, Schütz, Hönig, Schulz, Kern, Bommer, Schrezenmeier, Kirchhoff, Jahrsdörfer: "CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 8, pp. 3768-77, (2015) (PubMed).

French, Huard, Wysocka, Shane, Contassot, Arrighi, Piguet, Calderara, Rook: "Impaired CD40L signaling is a cause of defective IL-12 and TNF-alpha production in Sézary syndrome: circumvention by hexameric soluble CD40L." dans: Blood, Vol. 105, Issue 1, pp. 219-25, (2004) (PubMed).

Holler, Tardivel, Kovacsovics-Bankowski, Hertig, Gaide, Martinon, Tinel, Deperthes, Calderara, Schulthess, Engel, Schneider, Tschopp: "Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex." dans: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1428-40, (2003) (PubMed).

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40L

Sujet:

MultimericCD40L™, ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L

MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.

Poids moléculaire: ~35-40kDa (SDS-PAGE)

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints