CD40 Ligand Protein (CD40LG) (His tag)

N° du produit ABIN7320848

Cette protéine Recombinant CD40 Ligand est produite dans Human Cells.

Aperçu rapide pour CD40 Ligand Protein (CD40LG) (His tag) (ABIN7320848)

Antigène: CD40 Ligand (CD40LG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Human Cells

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CD40 Ligand protéine est marqué à la His tag.

Fonction: Recombinant Mouse CD40LG/TNFSF5 Protein (His Tag)(Active)

Séquence: Met112-Leu260

Attributs du produit: Recombinant Mouse CD40L is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human CD40-Fc(Cat: PKSM041225) at 2μg/ml(100 μl/well) can bind Human CD40LG-His. The ED50 of Human CD40LG-His(Cat: PKSH033600)) is 13.84ng/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40LG/TNFSF5

Sujet:

Background: CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.

Synonym: CD40 Ligand, CD40LG,HIGM1, T-B cell-activating molecule, T-BAM, TNFSF5, tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5

Poids moléculaire: 18.8 kDa

UniProt: P27548

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints