Beclin 1 Protein (AA 1-450) (GST tag)
(3 références)-
- Antigène Voir toutes Beclin 1 (BECN1) Protéines
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-450
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Origine
- Humain
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Source
- Wheat germ
- Purification/Conjugué
- Cette Beclin 1 protéine est marqué à la GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Fonction
- BECN1 (Human) Recombinant Protein (P01)
- Séquence
- MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK
- Attributs du produit
- Human BECN1 full-length ORF ( NP_003757.1, 1 a.a. - 450 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Beclin 1, Autophagy Related (BECN1) (AA 46-152) protein (His-IF2DI Tag)
BECN1 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 351-450) protein (GST tag)BECN1 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Beclin 1, Autophagy Related (BECN1) (AA 1-450) protein (His tag)BECN1 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 1-450) protein (Strep Tag)Crystallography grade BECN1 Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 1-448) protein (Strep Tag)Crystallography grade BECN1 Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 150-450) protein (His tag)Crystallography grade BECN1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 134-450) protein (His tag)Crystallography grade BECN1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 132-448) protein (His tag)Crystallography grade BECN1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 148-448) protein (His tag)Crystallography grade BECN1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) protein (Myc-DYKDDDDK Tag)BECN1 Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Conseil sur la manipulation
- Aliquot to avoid repeated freezing and thawing.
- Stock
- -80 °C
- Stockage commentaire
- Best use within three months from the date of receipt of this protein.
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: "High Beclin 1 expression defines a poor prognosis in endometrial adenocarcinomas." dans: Gynecologic oncology, Vol. 123, Issue 1, pp. 147-51, (2011) (PubMed).
: "Depletion of Beclin-1 due to proteolytic cleavage by caspases in the Alzheimer's disease brain." dans: Neurobiology of disease, Vol. 43, Issue 1, pp. 68-78, (2011) (PubMed).
: "Developmentally regulated ceramide synthase 6 increases mitochondrial Ca2+ loading capacity and promotes apoptosis." dans: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4644-58, (2011) (PubMed).
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: "High Beclin 1 expression defines a poor prognosis in endometrial adenocarcinomas." dans: Gynecologic oncology, Vol. 123, Issue 1, pp. 147-51, (2011) (PubMed).
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- Antigène
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- Autre désignation
- BECN1 (BECN1 Produits)
- Synonymes
- beclin-1 Protein, atg6 Protein, vps30 Protein, beclin1 Protein, Beclin-1 Protein, becn1 Protein, zgc:73097 Protein, ATG6 Protein, VPS30 Protein, 4921513J16Rik Protein, 5430417M23Rik Protein, Atg6 Protein, beclin 1 Protein, beclin 1, autophagy related Protein, beclin 1 protein Protein, beclin-1 Protein, beclin family protein Protein, beclin 1, autophagy related L homeolog Protein, BECN1 Protein, becn1 Protein, atg6 Protein, CpipJ_CPIJ011087 Protein, PTRG_07825 Protein, SJAG_01876 Protein, Becn1 Protein, becn1.L Protein
- Sujet
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Full Gene Name: beclin 1, autophagy related
Synonyms: ATG6,VPS30,beclin1 - ID gène
- 8678
- NCBI Accession
- NM_003766
- Pathways
- Autophagy
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