GAD65 Protein (AA 1-585) (GST tag)

N° du produit ABIN1354671

Détail du produit

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-585

Origine: Humain

Source: Wheat germ

Purification/Conjugué: Cette GAD65 protéine est marqué à la GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Fonction: GAD2 (Human) Recombinant Protein (P02)

Séquence: MASPGSGFWS FGSEDGSGDS ENPGTARAWC QVAQKFTGGI GNKLCALLYG DAEKPAESGG SQPPRAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MMIARFKMFP EVKEKGMAAL PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV PLQCSALLVR EEGLMQNCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV DVFKLWLMWR AKGTTGFEAH VDKCLELAEY LYNIIKNREG YEMVFDGKPQ HTNVCFWYIP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL

Attributs du produit: Human GAD2 full-length ORF ( NP_000809.1, 1 a.a. - 585 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Stockage

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Conseil sur la manipulation: Aliquot to avoid repeated freezing and thawing.

Stock: -80 °C

Stockage commentaire: Best use within three months from the date of receipt of this protein.

Référence

Grupillo, Gualtierotti, He, Sisino, Bottino, Rudert, Trucco, Fan: "Essential roles of insulin expression in Aire+ tolerogenic dendritic cells in maintaining peripheral self-tolerance of islet ?-cells." dans: Cellular immunology, Vol. 273, Issue 2, pp. 115-23, (2012) (PubMed).

Fan, Rudert, Grupillo, He, Sisino, Trucco: "Thymus-specific deletion of insulin induces autoimmune diabetes." dans: The EMBO journal, Vol. 28, Issue 18, pp. 2812-24, (2009) (PubMed).

Détail du antigène

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Autre désignation: GAD2 (GAD2 Produits)

Synonymes: GAD65 Protein, gad65 Protein, F12P19.12 Protein, F12P19_12 Protein, GLUTAMATE DECARBOXYLASE 2 Protein, glutamate decarboxylase 2 Protein, zgc:112198 Protein, 6330404F12Rik Protein, GAD(65) Protein, Gad-2 Protein, glutamate decarboxylase 2 Protein, glutamate decarboxylase 1 Protein, glutamic acid decarboxylase 2 Protein, GAD2 Protein, PTRG_06148 Protein, LOC9324534 Protein, Gad2 Protein, GAD1 Protein, gad2 Protein

Sujet: Full Gene Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65 kDa)
Synonyms: GAD65,MGC161605,MGC161607

ID gène: 2572

NCBI Accession: NM_000818