PDGF-AB Heterodimer (Homodimer) (Active) Protéine

N° du produit ABIN1589625

Détail du produit

Antigène: PDGF-AB Heterodimer

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Homodimer

Origine: Humain

Source: Escherichia coli (E. coli)

Séquence: Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT

Attributs du produit: Length (AA): 126/110
The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Pureté: > 95 % by SDS-PAGE. Visualized by silver stain

niveau d'endotoxine: < 0.1 ng per µg of PDGF-AB

Information d'application

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized PDGF-AB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1%) is recommended.

Buffer: 50 mM acetic acid

Conseil sur la manipulation: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: The lyophilized PDGF-AB is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted PDGF-AB is best stored at -20 °C to -70 °C.

Détail du antigène

Antigène: PDGF-AB Heterodimer

Autre désignation: PDGF-AB

Synonymes: PDGF-A Protein, PDGF1 Protein, platelet derived growth factor subunit A Protein, PDGFA Protein

Sujet: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).
Synonyms: PDGFA, PDGF1, PDGF-A

Poids moléculaire: 25.5 kDa

NCBI Accession: NP_002599, NM_002608, NP_002598, NM_002607

UniProt: P01127, P04085