IL-1 beta Protein (AA 118-269)

N° du produit ABIN2018012

Cette protéine Recombinant IL-1 beta est exprimée dans CHO Cells.

Aperçu rapide pour IL-1 beta Protein (AA 118-269) (ABIN2018012)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: CHO Cells

Pureté: > 95 % as analyzed by SDS-PAGE and HPLC.

Détail du produit

Attributs du protein: AA 118-269

Attributs du produit: ED50 < 10 pg/mL, measured in a cell proliferation assay using D10S cells, corresponding to a specific activity of > 1×10^8 units/mg.

niveau d'endotoxine: < 0.2 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant Murine Interleukin 1 beta (IL-1 beta) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmIL-1beta should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: Interleukin 1 beta (IL-1 Beta)

Sujet: Interleukin-1 (IL-1) is a family of cytokines that play a central role in the regulation of immune and inflammatory responses to infections or sterile insults. IL-1a and IL-1b are the first two members discovered in this family, which are the products of distinct genes recognizing the same cell surface receptors. IL-1a and IL-1b are structurally related polypeptides that show approximately 25 % homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-1b is interleukin 1b-converting enzyme (ICE)/caspase-1. Mature human and mouse IL-1b share approximately 75 % amino acid sequence identity where human IL-1b has been found to be active on murine cell lines. Interleukin (IL)-1b, murine, produced in CHO cells, is a non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17,400 Da.
Synonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

Poids moléculaire: 17.4 kDa, observed by non-reducing SDS-PAGE.

UniProt: P10749

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome