IL12 Protein (AA 23-219, AA 23-328)

N° du produit ABIN2018013

Détail du produit

Antigène: IL12 (Interleukin 12 (IL12))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 23-219, AA 23-328

Origine: Humain

Source: HEK-293T Cells

Attributs du produit: EC50 < 2 ng/mL, measured by the induction of IFN-gamma from NK cells co-stimulated with IL-18, corresponding to a specific activity of > 1x10^6 units/mg.
P35 subunit: AA 23-219 ,P40 subunit: AA 23-328

Pureté: > 95 % as analyzed by SDS-PAGE and HPLC.

niveau d'endotoxine: < 0.2 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant Human Interleukin 12(IL-12) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rh-IL12 should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: Interleukin 12(IL-12) (IL12 Produits)

Synonymes: CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein

Sujet: Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70 % and 60 % amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the mouse system.
Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF

Poids moléculaire: 75 kDa, consisting of a 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit.

UniProt: P29459, P29460

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome